A Simple Analysis Method for 4-Deoxy-l-erythro-5.
Determination of the Uronic Acid Content of Plant Cell Walls Using a Colorimetric Assay. Laurence D. Melton. University of Auckland, Auckland, New Zealand. Search for more papers by this author. Bronwen G. Smith. University of Auckland, Auckland, New Zealand. Search for more papers by this author. Laurence D. Melton.
The analysis method established in this study will contribute to the quantitative and qualitative analysis of DEH, and the activity measurement of exo-type alginate lyase. KW - 4-Deoxy-L-erythro-5-hexoseulose uronic acid. KW - Alginate. KW - Exo-type alginate lyase. KW - Falsirhodobacter sp. alg1.
Uronic Acid The compound obtained by replacing the hydroxymethyl group (CH 2 OH) in the molecule of an aldose with a carboxylic acid group is called a uronic acid. eg.
GO ID GO:0006063 Aspect Biological Process Description The chemical reactions and pathways involving uronic acid, any monocarboxylic acid formally derived by oxidizing to a carboxyl group the terminal hydroxymethylene group of either an aldose with four or more carbon atoms in the molecule, or of any glycoside derived from such an aldose.
Uronic acids are a class of sugar acids with both the carbonyl and the carboxylic acid functional groups. Uronic acids are present in plant cell walls and are also formed during biofuel processing.
Histochemical and biochemical studies have shown that the otoconial membrane, as well as the tectorial membrane and the cupula, contains glycoproteins and proteoglycans. However, uronic acids, which are essential elements of glycosaminoglycans (GAGs), have not so far been directly detected or quantitatively measured in inner ear samples.
Estimation of uronic acids using DMP corroborated the results of GLC analysis. The study evaluated the utility of colorimetric methods for uronic acid estimation in polysaccharide having interferences from neutral sugars especially xylose, glucose and galactose.